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The influence of skin barrier disruption and melanin content on the formation of DNA lesions and radicals in ex vivo human skin induced by 233 nm far-UVC irradiation from LEDs

L. Busch1, J. Schleusener1, D.F. Zamudio Díaz1, M. Kröger1, S.B. Lohan1, P. Zwicker2, S. Einfeldt3, M. Kneissl4, A.A. Kühl5, C. Witzel6, H. Klose7, C.M. Keck8, A. Kramer2, M.C. Meinke1

Published in:

Free Radical Biol. Med., vol. 189, suppl. 1, pp. 30-31 (2022).

Abstract:

As reported recently, 233 nm radiation emitted by a spectrally pure UVC LED source shows sufficient bactericidal properties at an applied dose between 20 and 80 mJ/cm2. In ex vivo human skin and skin models, the formation of epidermal DNA lesions at bactericidal doses was minor compared to one tenth of the minimal erythema dose of UVB light. This can be attributed to the strong absorption for wavelengths below 240 nm in the upper non-nucleated dermal cell layers. Furthermore, the radical formation was far lower than for a dose equivalent to a stay of 20 min outdoors, which can be compensated by the antioxidant defense system.

1 Center of Experimental and Applied Cutaneous Physiology, Department of Dermatology, Venerology and Allergology, Charité - Universitätsmedizin Berlin, Germany
2 Universitätsmedizin Greifswald, Institute of Hygiene and Environmental Medicine, Greifswald, Germany
3 Ferdinand-Braun-Institut, Berlin, Germany
4 Technische Universität Berlin, Institute of Solid State Physics, Berlin, Germany
5 Charité - Universitätsmedizin Berlin, iPATH.Berlin-Immunopathology for Experimental Models, Berlin, Germany
6 Charité - Universitätsmedizin Berlin, Division of Plastic and Reconstructive Surgery, Berlin, Germany
7 artMED Private Praxis for Plastic and Aesthetic Surgery, Berlin, Germany
8 Philipps-Universität Marburg, Department of Pharmaceutics and Biopharmaceutics, Marburg, Germany

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