High-resolution imaging techniques using fluorescent dyes are used in the biomedical field. For such applications, we are developing the suitable laser sources with an emission wavelength around 561 nm. These are based on high-power diode lasers in the wavelength range at 1122 nm, whose radiation is converted into the visible wavelength range by means of nonlinear frequency conversion. Depending on the requirements of the respective process (STED, confocal microscopy, etc.), we optimize the modules in different power classes - from 10 mW to 2 W and for pulsed or continuous wave operation.