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A UV-C LED-based unit for continuous decontamination of the sheath fluid in a flow-cytometric cell sorter

J. Kirsch1, K. Heinrich1, D. Kage1, J. Glaab2, B. Grothe3, K. v. Volkmann3, T. Kaiser1

Published in:

Eng. Life Sci., vol. 22, no. 8, pp. 550-553, doi:10.1002/elsc.202200025 (2022).

Abstract:

Aseptic cell sorting is challenging, especially when a flow-cytometric cell sorter is not operated in a sterile environment. The sheath fluid system of a cell sorter may be contaminated with germs such as bacteria, yeasts, viruses, or fungi. Thus, a regular chemical cleaning procedure is required to prepare a sorter for aseptic cell sorting by flushing the fluidic system. However, this procedure is time consuming, and most importantly, the researcher can never be sure that the cleaning process was successful. Here we present a method in which the sheath fluid of a cell sorter was decontaminated by irradiation with UV-C light using a flow- through principle. Using this principle, we were able to achieve a 5 log reduction of bacteria in the sheath fluid.

1 Flow Cytometry Core Facility, Deutsches Rheuma-Forschungszentrum, ein Institut der Leibniz-Gemeinschaft, Berlin, Germany
2 Ferdinand-Braun-Institut gGmbH, Leibniz-Institut für Höchstfrequenztechnik, Berlin, Germany
3 APE Angewandte Physik und Elektronik GmbH, Berlin, Germany

Keywords:

aseptic sorting, flow-cytometric cell sorting, UV-C LEDs

Copyright © 2022 The Authors. Engineering in Life Sciences published by Wiley-VCH GmbH.
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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